Author(s):
1. Valentina Veselinović, Medicinski fakultet Banja Luka , Republic of Srpska, Bosnia and Herzegovina
2. Nataša Trtić, Medicinski fakultet Banja Luka , Republic of Srpska, Bosnia and Herzegovina
3. Tijana Adamovic, University of Banja Luka, School of Medicine, Dentistry Study Program, Republic of Srpska, Bosnia and Herzegovina
4. Olivera Dolić, Medicinski fakultet Banja Luka , Republic of Srpska, Bosnia and Herzegovina
5. Radmila Arbutina, University of Banja Luka, School of Medicine, Dentistry Study Program, Republic of Srpska, Bosnia and Herzegovina
6. Aleksandra Đeri, Medicinski fakultet Banja Luka , Republic of Srpska, Bosnia and Herzegovina
7. Saša Marin, Medicinski fakultet Banja Luka , Republic of Srpska, Bosnia and Herzegovina
8. Nataša Knežević, Medicinski fakultet Banja Luka , Republic of Srpska, Bosnia and Herzegovina

Abstract:
Purpose. The gingivally supported denture base, made of conventional polymethyl methacrylate (PMMA), is susceptible to biofilm formation and the development of denture stomatitis. The purpose of this work is the production of PMMA enriched with gold nanoparticles (AuNPs) with improved antimicrobial properties. Material and methods. AuNPs were added to denture base PMMA in concentration of 0,5% and 1%. Heat-polymerized acrylic resin specimens of pure PMMA and nanomodified PMMA (PMMA/AuNPs) were prepared according to each test specification. Surface roughness and surface free energy were measuremed by Profilomethar, and hydrofobicity and contact angle were measured by Tensiometar. Antimicrobial activity of newly formed PMMA/AuNPs were evaluated for two different microbial streins (Candidae albicans and Staphylococcus aureus) in monomicrobial biofilm form. Colony forming units (CFUs), cell metabolic activity (MTT) and disc diffusion test for each tested strain were performed. Results: The surface roughness, surface free energy increase in PMMA/AuNPs samples. There is no statisticaly differences between tested groups. Hidrofobicity and contact angle measurement shows differences compare to unmodified PMMA. PMMA/AuNPs showed a significant reduction in the monomicrobial biofilms of all tested species compare to control group and it is in direct coleration with percentage of added AuNPs. The CFUs test of AuNPs modified PMMA samples did not show change compare to pure PMMA. Released AuNPs did not recorded around the PMMA/AuNPs samples. Conclusion: Incorporation of AuNPs into heat-polymerised denture base PMMA led to surface properties change. Antimicrobial activity of PMMA/AuNPs increase in corelation with percentage of AuNPs and it is significantly expressed even in a very small concentration of gold nanoparticles.

Key words:
gold nanoparticles,denture base,acrylic resin,denture stomatitis

Thematic field:
SYMPOSIUM B - Biomaterials and nanomedicine

Date of abstract submission:
31.08.2023.

Conference:
Contemporary Materials 2023 - Savremeni materijali

Abstract