Author(s):
1. Ivan Djuricic, Mašinski fakultet Univerziteta u Beogradu, Serbia
2. Jovana Šakota-Rosić, Mašinski fakultet Univerziteta u Beogradu, Serbia
3. Ivana Stanković (Mileusnić), Mašinski fakultet Univerziteta u Beogradu, 4. Jelena Munćan, Mašinski fakultet Univerziteta u Beogradu, Serbia
5. Dušan Šarac, Univeristy of Belgrade, Faculty of Mechanical Engineering, Serbia
6. Boris Kosić, Mašinski fakultet Univerziteta u Beogradu, Serbia
7. Lidija Matija, Mašinski fakultet Univerziteta u Beogradu, Serbia

Abstract:
Fourier transformed infrared spectroscopy of human serum albumin (HSA) in the region of 650-4000cm-1 was subjected for developing reagentless measurement of HSA. The infrared spectra were subjected to partial least squares (PLS) regression to obtain calibration models predicting the concentrations of the HSA. The calibration in the concentration of 1 - 6.5mg/dl has yielded a correlation coefficient of 0.99 and a standard error of prediction of 0.11mg/dl with latent variable of six. These result suggests that the infrared spectra of aqueous solutions of HSA and its calibration model developed using PLS regression can extract up effectively the information about albumin contained in water. These results provide a base for development of reagentless method of measurement of HSA in human serum and also in vivo HSA measurement.

Key words:
human serum albumi,FTIR-ATR,PLS regression

Thematic field:
SYMPOSIUM C - Water

Date of abstract submission:
14.06.2015.

Conference:
Contemporary Materials 2015 - Savremeni Materijali

Abstract